解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Reduction of optical scattering in turbid biological tissues using nonreactive chemical agents has potential applications for light-based diagnostics and therapeutics. Optical clearing effects by exogenous chemical agents, in particular sugars and sugar alcohols, have been found to be temporary with tissue rehydration...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2166381
更新日期:2006-01-01 00:00:00
abstract::A transgenic mouse model with a Clim [co-factor of LIM (a combination of first letters of Lin-11 (C. elegans), ISL1 (rat), and Mec-3 (C. elegans) gene names) domain proteins] gene partially blocked in the epithelial compartment of its tissues is used to establish the sensitivity of intrinsic reflectance nonlinear opti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2163254
更新日期:2006-01-01 00:00:00
abstract::We introduce the concept of deliberate placement of absorbers to alter the average path of photons through tissue for a biomedical optical device. By changing the reflectivity of a needle that separates a source and detector, the average photon path through a turbid medium can be changed. Totally reflective needles ha...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2168167
更新日期:2006-01-01 00:00:00
abstract::A miniature fiber optic spectrometer enclosed within a semipermeable (dialysis) membrane is proposed for in vivo interstitial sensing applications. The semipermeable membrane acts as a molecular filter, allowing only small molecules to pass through to the sampling volume. This filtering, in principle, should enable co...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2166086
更新日期:2006-01-01 00:00:00
abstract::Fourier domain (FD) techniques have increasingly gained attention in optical coherence tomography (OCT). This is primarily due to their demonstrated sensitivity of two to three orders of magnitude over conventional time-domain techniques. FDOCT images are subject to two primary sources of artifacts. First, a complex c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2136147
更新日期:2005-11-01 00:00:00
abstract::Prolonged excitation of fluorescent probes leads eventually to loss of their capacity to emit light. A decrease in the number of detected photons reduces subsequently the resolving power of a fluorescence microscope. Adverse effects of fluorescence intensity loss on the quality of microscopic images of biological spec...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2136313
更新日期:2005-11-01 00:00:00
abstract::We investigate the statistical polarization parameters of biological tissue histological section images with different morphological structure. First we outline the results of polarization coordinate mapping and analysis of the statistics of the first to fourth orders of biological tissue image polarization azimuth an...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2148251
更新日期:2005-11-01 00:00:00
abstract::Many optical diagnostic approaches rely on changes in scattering and absorption properties to generate optical contrast between normal and diseased tissue. Recently, there has been increasing interest in using exogenous agents to enhance this intrinsic contrast with particular emphasis on the development for targeting...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2141825
更新日期:2005-11-01 00:00:00
abstract::High-speed (video-rate) fluorescence lifetime imaging (FLIM) through a flexible endoscope is reported based on gated optical image intensifier technology. The optimization and potential application of FLIM to tissue autofluorescence for clinical applications are discussed. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2102807
更新日期:2005-09-01 00:00:00
abstract::The earliest steps of breast cancer begin with aberrations in mammary ductal structure. Techniques that enable an investigator to image in situ and then analyze the same tissue using biochemical tools facilitates identification of genetic networks and signaling pathways active in the imaged structure. Cellular confoca...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2065827
更新日期:2005-09-01 00:00:00
abstract::Compact optical imagers that can detect both spectral and polarization signatures are required in many biomedical applications. An acousto-optic-tunable-filter (AOTF)-based imager is ideally suited to provide both agile spectral and polarization signatures. Such an imager can be readily used for real-time in vivo medi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2102507
更新日期:2005-09-01 00:00:00
abstract::The cyclic peptide, cyclopentapeptide cyclo(lys-Arg-Gly-Asp-phe) (c(KRGDf)), which is known to target alpha(v)beta3 integrin, is dual-labeled with a radiotracer, (111)indium, for gamma scintigraphy as well as with a near-infrared dye, IRDye800, for continuous-wave (cw) imaging of alpha(v)beta3 positive human M21 melan...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2114748
更新日期:2005-09-01 00:00:00
abstract::The effect of sampling region size and tissue heterogeneity is examined using fluorescence histogram assessment in a rat prostate tumor model with benzoporphyrin derivative fluorophore. Spatial heterogeneity in the fluorescence signal occurs on both macroscopic and microscopic scales. The periphery of the tumor is mor...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2002978
更新日期:2005-07-01 00:00:00
abstract::Raman spectroscopy is an optical technique able to interrogate biological tissues, giving us an understanding of the changes in molecular structure that are associated with disease development. The Kerr-gated Raman spectroscopy technique uses a picosecond pulsed laser as well as fast temporal gating of collected Raman...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.1991848
更新日期:2005-07-01 00:00:00
abstract::Computer simulation is used to facilitate the design of fiber-probe geometries that enable enhanced detection of optical signals arising from specific tissue depths. Obtaining understanding of the relationship between fiber-probe design and tissue interrogation is critical when developing strategies for optical detect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1989335
更新日期:2005-07-01 00:00:00
abstract::We describe the protocol for an inexpensive and nondestructive optical reflectance assay for the measurement of biofilm formation. Reflectance data are obtained using an Ocean Optics (Dunedin, Florida) USB 2000 spectrometer with a polychromatic light source. A fiber optic cable is used both for illumination and collec...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1953347
更新日期:2005-07-01 00:00:00
abstract::The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determine...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1922329
更新日期:2005-05-01 00:00:00
abstract::We describe the use of a Teflon-AF liquid core optical fiber (LCOF) geometry to enhance the collection of Raman scattering from the biochemical creatinine, dissolved in water and in urine. At short integration times, where shot noise is most troublesome, the enhanced signal leads to greater accuracy in estimating the ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1917842
更新日期:2005-05-01 00:00:00
abstract::Multiphoton microscopy has evolved into a powerful bioimaging tool in three dimensions. However, the ability to image biological specimens in-depth can be hindered by sample spherical aberration and scattering. These two phenomena can result in the degradation of image resolution and the loss of detected multiphoton s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1924614
更新日期:2005-05-01 00:00:00
abstract::A simple empirical method is presented to determine the scattering coefficient mu' s from backscattered polarized images of turbid media. It uses the ratio, pixel by pixel, of two images that are the second and the first backscattered Stokes parameter images Q and I, respectively. Taking this image ratio, then integra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1924715
更新日期:2005-05-01 00:00:00
abstract::Photobiological research in the last 30 yr has shown the existence of ultraweak photon emission in biological tissue, which can be detected with sophisticated photomultiplier systems. Although the emission of this ultraweak radiation, often termed biophotons, is extremely low in mammalian cells, it can be efficiently ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1899185
更新日期:2005-03-01 00:00:00
abstract::Spectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1891370
更新日期:2005-03-01 00:00:00
abstract::We have employed near-infrared optical methods to measure noninvasively the dynamics of muscle blood flow and oxygen saturation (StO2) during cuff occlusion and plantar flexion exercise. Relative muscle oxygen consumption (rVO2) was also computed from these data. Diffuse correlation spectroscopy provides information a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1884603
更新日期:2005-03-01 00:00:00
abstract::We present functional imaging of the concentration of a photodynamic therapy (PDT)-related dye in scattering tissue phantoms based on spatially resolved measurements of optical properties through spectroscopic optical coherence tomography (OCT). Expressions for the OCT signal are developed, enabling estimation of dept...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1898242
更新日期:2005-03-01 00:00:00
abstract::Cortical spreading depression (CSD) is a pronounced depolarization of neurons and glia that spreads slowly across the cortex followed by a period of depressed electrophysiological activity. The vascular changes associated with CSD are a large transient increase in blood flow followed by a prolonged decrease lasting gr...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1852556
更新日期:2005-01-01 00:00:00
abstract::Diffuse optical imaging is an effective technique for noninvasive functional brain imaging. However, the measurements respond to systemic hemodynamic fluctuations caused by the cardiac cycle, respiration, and blood pressure, which may obscure or overwhelm the desired stimulus-evoked response. Previous work on this pro...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1852552
更新日期:2005-01-01 00:00:00
abstract::Penetration profiles of topically applied drugs and cosmetic products provide important information on their efficacy. The application of tape stripping in combination with UV/VIS spectroscopy is checked to determine the local position of topically applied substances inside the stratum corneum, the penetration profile...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1854683
更新日期:2005-01-01 00:00:00
abstract::We successfully acquire the in vivo Raman spectrum of melanin from human skin using a rapid near-infrared (NIR) Raman spectrometer. The Raman signals of in vivo cutaneous melanin are similar to those observed from natural and synthetic eumelanins. The melanin Raman spectrum is dominated by two intense and broad peaks ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1805553
更新日期:2004-11-01 00:00:00
abstract::We investigate the relationship between the laser beam at the retina (spot size) and the extent of retinal injury from single ultrashort laser pulses. From previous studies it is believed that the retinal effect of single 3-ps laser pulses should vary in extent and location, depending on the occurrence of laser-induce...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1805554
更新日期:2004-11-01 00:00:00
abstract::Amblyopia is a form of visual impairment caused by ocular misalignment (strabismus) or defocus in an otherwise healthy eye. If detected early, the condition can be fully treated, yet over half of all children with amblyopia under age 5 escape detection. We developed a Pediatric Vision Screener (PVS) to detect amblyopi...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.1805561
更新日期:2004-11-01 00:00:00
abstract::Quantitative microscopy has been extensively used in biomedical research and has provided significant insights into structure and dynamics at the cell and tissue level. The entire procedure of quantitative microscopy is comprised of specimen preparation, light absorption/reflection/emission from the specimen, microsco...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.1779233
更新日期:2004-09-01 00:00:00
abstract::Blood, being a suspension of deformable red cells suspended in plasma, displays flow dynamics considerably more complicated than those of an ideal Newtonian fluid. Flow dynamics in blood capillaries of a few hundred micrometers in diameter are investigated using Doppler optical coherence tomography (DOCT) and Doppler ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1781163
更新日期:2004-09-01 00:00:00
abstract::A computer simulation called RHME (Retinal Heating in Moving Eye) is developed to simulate the heating pattern that occurs in the retina during a long-duration exposure to a continuous wave laser beam. The simulation takes into account eye movements that occur during a deliberate fixation. Due to the rapid (millisecon...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1783355
更新日期:2004-09-01 00:00:00
abstract::We present a novel confocal microscope that has dual-axis architecture and biaxial postobjective scanning for the collection of fluorescence images from biological specimens. This design uses two low-numerical-aperture lenses to achieve high axial resolution and long working distance, and the scanning mirror located d...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1760760
更新日期:2004-07-01 00:00:00
abstract::The results of an experimental study of the possibilities of monitoring erbium yttrium aluminum garnet laser-mediated ablation of human epidermis with the use of Fourier transform infrared (FTIR) spectroscopy and spectral polarization techniques are presented. The attenuated total reflection (ATR) method was used for ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1755719
更新日期:2004-07-01 00:00:00
abstract::We describe the use of a biochip based on complementary metal oxide semiconductor (CMOS) technology for detection of specific genetic sequences using molecular beacons (MB) immobilized on solid surfaces as probes. The applicability of this miniature detection system for screening for the BRCA1 gene is evaluated using ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1691025
更新日期:2004-05-01 00:00:00
abstract::Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure ...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.1695562
更新日期:2004-05-01 00:00:00
abstract::We present a new detection instrument for sensor measurements based on excited-state fluorescence lifetimes. This system consists of a primary optoelectronic loop containing a resonance-type rf amplifier, a modulatable fluorescence-excitation light source, a fiber optic feedback loop (with a gap for a fluorescent sens...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1688814
更新日期:2004-05-01 00:00:00
abstract::Optical coherence tomography (OCT) provides more parameters than pure morphology does. In a recent paper [A. Knuettel and M. Boehlau-Godau, J. Biomed. Opt. 5(1) 83-92 (2000)] we have shown that the refractive index (RI) can be evaluated in a localized manner in skin tissue under in vivo conditions. Based on a theory, ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1647544
更新日期:2004-03-01 00:00:00
abstract::Fluorescence diagnostic techniques are notable amongst many other optical methods because they offer high sensitivity and noninvasive measurement of tissue properties. However, a combination of multiple scattering and physical heterogeneity of biological tissues hampers interpretation of the fluorescence measurements....
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1645796
更新日期:2004-03-01 00:00:00